A Mini Review on the Synthesis of Ag-Nanoparticles by Chemical Reduction Method and their Biomedical Applications


  • Qurbat Zahra
  • Ahmad Fraz
  • Almas Anwar
  • Muhammad Awais
  • Mudassar Abbas Associate Professor, School of Textile and Design, University of Management and Technology, C-II Johar Town Lahore 54770, Pakistan.




Antimicrobial properties, Blended materials, Green synthesis, Silver nanoparticles


This mini-review provides an adequate amount of information about the synthesis of silver nanoparticles through various routes. Among the competitors, Sodium-, borohydride and citrate, water, polyvinyl pyrrolidone and sodium citrate and silver nitrate are the most commonly employed reducing agents, solvent, capping agents and silver salt precursor. The particles thus formed when incorporated to form blended materials can be used in advanced applications, especially in medical devices, preventing the adhesion of microorganisms over them. Herein, the similar function of silver, either distributed over the polymeric material surfaces as a polished layer or equally dispersed therein, will be presented. The marked examples include incorporation of silver nanoparticles into fluff pulp through sonochemical impregnation and blending the silver nanoparticles synthesized through solvent free, greener routes into poly(dicyclopentadiene).

Author Biography

Mudassar Abbas, Associate Professor, School of Textile and Design, University of Management and Technology, C-II Johar Town Lahore 54770, Pakistan.

Dr. Mudassar Abbas obtained his MSc degree from Punjab University Lahore after graduating from FC College University, Lahore with distinction. During his postgraduate studies, the major areas of research were synthesis and studies of acid dyes and polyvinylacetate. Keeping in view the demands and practical application of these materials in industry, he started his professional carrier from textile industry. Having gained one year industrial experience, he then diversified as an academic professional and taught chemistry at various levels. In 2008, he went to Graz, Austria to pursue his PhD in technical Chemistry and was awarded with a doctoral degree in Natural Sciences (Dr. rer. nat.) with distinction. During his PhD, he participated in many educational activities, conferences, and meetings, where he scientifically contributed with his writings and engaged in interdisciplinary work. After completing his PhD, he continued his research as a Post-Doctoral research fellow in the same group.



A. Lucas, “Silver in ancient times”, J. Egypt. Arch., 14, 313 (1928).

T. M. Tolaymat, A. M. E. Badawy, A. Genaidy, K. G. Scheckel, T. P. Luxton and M. Suidan, “An evidence-based environmental perspective of manufactured silver nanoparticle in syntheses and applications: A systematic review and critical appraisal of peer-reviewed scientific papers”, Sci. Total Environ., 408, 999 (2010).

Q. Li, S. Mahendra, D. Y. Lyon, L. Brunet, M. V. Liga and D. Li, “Antimicrobial nanomaterials for Water Disinfection and Microbial Control: Potential Applications and Implications”, Water Res., 42, 4591 (2008a).

S. A. Blaser, M. Scheringer, M. MacLeod and K. Hungerbuhler, “Estimation of cumulative aquatic exposure and risk due to silver: contribution of nano-functionalized plastics and textiles”, Sci. Total Environ., 390, 396 (2008).

F. Furno, K. S. Morley, B. Wong, B. L. Sharp, P. L. Arnold and S. M. Howdle, et al., Silver nanoparticles and polymeric medical devices: a new approach to prevention of infection, J Antimicrob Chemother., 54, 1019 (2004).

L. Braydich-Stolle, S. Hussain, J. J. Schlager and M. C. Hofmann, . In vitro cytotoxicity of nanoparticles in mammalian germline stem cells, Toxicol. Sci., 88, 412 (2005).

N. Perkas, G. Amirian, S. Dubinsky, S. Gazit and A. Gedanken, Ultrasound-assisted coating of nylon 6,6 with silver nanoparticles and its antibacterial activity, J of Appl Polym Sci., 104, 1423 (2007).

A. B. A. Boxall, Q. Chaudhry, C. Sinclair, A. Jones, R. Aitken and B. Jefferson, et al. Current and future predicted environmental exposure to engineered nanoparticles, Sand Hutton, UK: Central Science Laboratory, (2007).

W. W. De, T. Vrecauteren and W. Verstraete, Method for production metal nanoparticle, Patent-WO2008003522 (2008).

S. Iravani, H. Korbekandi, S. V. Mirmohammadi, B. Zolfaghari, “Synthesis of silver nanoparticles: chemical, physical and biological methods”, Research in Pharmaceutical Sciences, 9, 385, (2014).

K. Toisawa, Y. Hayashi, H. Takizawa, “Synthesis of highly concentrated Ag nanoparticles in a heterogeneous solid-liquid system under ultrasonic irradiation”, Materials Transactions, 51, 1764, (2010).

A. B. Smetana, K. J. Klabunde, C. M. Sorensen, “Synthesis of spherical silver nanoparticles by digestive ripening, stabilization with various agents, and their 3-D and 2-D superlattice formation”, J. Colloid. Interface Sci. 284, 521, (2005).

X. Chen and H. J. Schluesener, Nanosilver: a nanoproduct in medical applications, Toxicol Lett., 176, 1 (2008).

S. Si and T. K. Mandal, Tryptophan-based peptides to synthesize gold and silver nanoparticles: a mechanistic and kinetic study, Chem Eur J., 13, 3160 (2007).

A. Y. Olenin, Y. A. Krutyakov, A. A. Kudrinskii and G. V. Lisichkin, Formation of surface layers on silver nanoparticles in aqueous and water-organic media, Colloid J., 70, 71 (2008).

J. Yang, j. y. Lee and H. Too, Core-shell Ag–Au nanoparticles from replacement reaction in organic medium, J Phys Chem B, 109, 19208 (2005).

L. Guo, J. Nie, B. Du, Z. Peng, B. Tesche and K. Kleinermanns, Thermoresponsive polymerstabilized silver nanoparticles, J Colloid Interface Sci., 319, 175 (2008).

M. G. Guzman, J. Dille and S. Godet, Synthesis of silver nanoparticles by chemical reduction method and their antimicrobrial activity, Proceedings of World Academy of Science, Engineering and Technology, 43, 357 (2008).

M. Abbas, A. Leigeb, J. Kienberger and C. Slugovc C. Solvent-free synthesis of silver-nanoparticles and their use as additive in poly(dicyclopentadiene), Journal of Chemical Soc of Pakistan., 35, 359 (2013).

J. He and T. Kunitake, Formation of silver nanoparticles and nanocraters on silicon wafers, Langmuir., 22, 7881, (2006).

W. Wang, S. Efrima and O. Regev, Directing silver nanoparticles into colloid-surfactant lyotropic lamellar systems, J. Phys. Chem. B., 103, 5613 (1999b).

S. D. Solomon, M. Bahadory, A. V. Jeyarajasingam, S. A. Rutkowsky and C. Boritz, Synthesis and study of silver nanoparticles, J. Chem. Educ., 84, 322 (2007).

D. Seo, W. Yoon, S. Park and J. Kim, The preparation of hydrophobic silver nanoparticles via solvent exchange method, Colloids Surfaces A., 313, 158 (2006).

Y. Song, W. Yanga and M. King, Shape controlled synthesis of sub-3 nm Ag nanoparticles and their localized surface plasmonic properties, Chem. Phy. Lett., 455, 218 (2008).

B. K. Kuila, A. Garai and A. K. Nandi, Synthesis, optical, and electrical characterization of organically soluble silver nanoparticles and their poly(3-hexylthiophene) nanocomposites: enhanced luminescence property in the nanocomposite thin films, Chem. Mater., 19, 5443 (2007).

J. Yang, J. Y. Lee and H. Too, A general phase transfer protocol for synthesizing alkylamine-stabilized nanoparticles of noble metals, Anal. Chem. Acta., 588, 34 (2007).

L. C. Courrol, F. R. De Oliveira Silva and L. Gomes . A simple method to synthesize silver nanoparticles by photo-reduction, Colloids Surfaces A., 305, 54 (2007).

K. Kalimuthu, R. S. Babu, D. Venkataraman, M. Bilal and S. Gurunathan Biosynthesis of silver nanocrystals by Bacillus licheniformis. Colloid Surfaces B., 65, 150 (2008).

S. Li, Y. Shen, A. Xie, X. Yu, L. Qiu, L. Zhang and Q. Zhang, Green synthesis of silver nanoparticles using Capsicum annuum L. extract, Green Chem., 8, 852 (2007).

N. Vigneshwaran, R. P. Nachane, R. H. Balasubramanya and P. V. Varadarajan, A novel one-pot ‘green’ synthesis of stable silver nanoparticles using soluble starch, Carbohyd. Res., 341, 2012 (2006).

S. Panigrahi, S. Kundu, S. K. Ghosh, S. Nath and T. Pal, Sugar assisted evolution of mono- and bimetallic nanoparticles, Colloid Surfaces A., 264, 133 (2005).

J. Zhang and J. R. Lakowicz, A model for DNA detection by metal-enhanced fluorescence from immobilized silver nanoparticles on solid substrate, J. Phys. Chem. B, 110, 2387 (2006).

M. N. Nadagouda and R. S. Varma, Green synthesis of Ag and Pd nanospheres, nanowires, and nanorods using vitamin B2: catalytic polymerisation of aniline and pyrrole. J. Nanomater., 782358 (2008).

J. Chen, J. Wang, X. Zhang and Y. Jin, Microwave-assisted green synthesis of silver nanoparticles by carboxymethyl cellulose sodium and silver nitrate, Mater. Chem. Phys., 108, 421 (2008c).

Y. Chen and X. Wang, Novel phase-transfer preparation of monodisperse silver and gold nanoparticles at room temperature. Mater. Lett., 62, 2215 (2008).

D. Wang, T. Xie, Q. Peng and Y. Li, Ag, Ag2S, and Ag2Se Nanocrystals: synthesis, assembly, and construction of mesoporous structures. J. Am. Chem. Soc., 130, 4016 (2008).

J. Bregado-Gutierrez, A. J. Saldıvar-Garcıa and H. F. Lopez, Synthesis of Silver Nanocrystals by a Modified Polyol Method. J. Appl. Polym. Sci., 107, 45 (2008).

G. W. Sławinski and F. P. Zamborini, Synthesis and alignment of silver nanorods and nanowires and the formation of Pt, Pd, and core/shell structures by galvanic exchange directly on surfaces, Langmuir., 23, 10357 (2007).

T. Hasell, J. Yang, W. Wang, P. D. Brown and S. M. Howdle, A facile synthetic route to aqueous dispersions of silver nanoparticles, Mater. Lett., 61, 4906 (2007).

T. Li, H. G. Park and S. Choi, . γ-Irradiation-induced preparation of Ag and Au nanoparticles and their characterizations, Mater. Chem. Phys., 105, 325 (2007).

S. Navaladian, B. Viswanathan, T. K. Varadarajan and R. P. Viswanath, Microwave-assisted rapid synthesis of anisotropic Ag nanoparticles by solid state transformation, Nanotechnology, 19, 1 (2008).

M. Abbas, S. Shafqat and C. Munir, Sonochemical impregnation of silvernanoparticle into fluff pulp and its antimicrobial efficacies, Nust J. Engg. Sci., 6, 10 (2013).

A. Panácek, L. Kvítek, R. Prucek, M. Kolár, R. Vecerová and N. Pizúrová, et al. Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity, J Phys Chem B, 110, 16248 (2006).

S. Pal, Y. K. Tak and J. M. Song, Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli, Appl Environ Microbiol., 73, 1712 (2007).

K. Kawahara, K. Tsuruda, M. Morishita and M. Uchida, Antibacterial effect of silver–zeolite on oral bacteria under anaerobic conditions, Dent Mater., 16, 452 (2000).

L. A. Casemiro, C. H. G. Martins, F. C. P. Pires-de-Souza and H. Panzeri, Antimicrobial and mechanical properties of acrylic resins with incorporated silver–zinc zeolite–Part 1, Gerodontology, 25, 187 (2005).

H. Kong and J. Jang, Antibacterial properties of novel poly(methyl methacrylate) nanofiber containing silver nanoparticles, Langmuir, 24, 2051 (2008).

G. Woods, Polyurethanes, materials, processing and applications. Rapra Review Reports. Report No. 15.Rapra Technology Ltd. 15 (1988).

A. Leitgeb, J. Wappel and C. Slugovc, The ROMP toolbox upgraded, Polymer, 51, 2927 (2010).

J. Kienberger, N. Noormofidi, I. Mühlbacher, I. Klarholz, C. Harms and C. Slugovc, Antimicrobial Equipment of poly(Isoprene) Applying Thiol-ene Chemistry, J. Polym. Sci. Part A: Polymer Chemistry, 50, 2236 (2012).

Md. S. A. S. Shah, M. Nag, T. Kalagara, S. Singh and S. V. Manorama, Silver on PEG-PU-TiO2 Polymer Nanocomposite Films: An Excellent System for Antibacterial Applications, Chemical Materials, 20,7, 2455, (2008)

D. J. Blazs, K. Triandafillu, P. Wood, Y. Chevelot, H. Harms, etal, "Inhibition of bacterial adhesion on PVC endotracheal tubes by RF oxygen glow discharge, Sodium hydroxide and silver nitrate treatments," Biomaterials, vol. 25, pp. 2139-2159, 2004.

J. W. Kim, J. E. Lee, J. H. zryu, J. S. Lee, S. J. Kim, S. H. Han, et al, "Synthesis of Metal/Polymer Colloidal Composites by the Tailored Deposition of Silver onto Porous Polymer Microspheres," Journal of Polymer Science: Part A: Polymer Chemistry, vol. 42, pp. 2551-2557, 2004.

T. T. T. Nguyen, B. Tae and J. S. Park, Synthesis and characterization of nanofiber webs of chitosan/ poly(vinyl alcohol) blends incorporated with silver nanoparticles, Journal of Material Science 46, 6528, (2011).

R. Pacios, R. Marcilla, C. Pozo-Gonzalo, J. A. Pomposo, H. Grande, J. Aizpurua and D. Mecerreyes, Combined electrochromic and plasmonic optical responses in conducting polymer/metal nanoparticle films, Journal of Nanoscience Nanotechnology, 7, 8, 2938, (2007)

H. Stara, Z. Stary and H. Munstedt, Silver Nanoparticles in Blends of Polyethylene and a Superabsorbent Polymer: Morphology and Silver Ion Release, Macromolecular Material Engineering , 296, 423, (2011)

M. A. Martínez-Rodríguez, M. A. Garza-Navarroa, I. E. Moreno-Corteza, R. Lucio-Porto and V. A. González, Silver/polysaccharide-based nanofibrous materials synthesized fromgreen chemistry approach, Carbohydrate Polymers, 136 46, (2016)

F. M. Kelly and J. h. Johnston, Colored and Functional Silver Nanoparticle_Wool Fiber Composites, ACS Applied Material Interfaces, 3, 1083, (2011)






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